Comparative analysis of swine leukocyte antigen gene diversity in Göttingen Minipigs

作者：Sabine E. Hammer, Tereza Duckova, Monica Gociman, Sandra Groiß, Clara P. S. Pernold, Karolin Hacker, Lena Kasper, Julia Sprung, Maria Stadler, Andres Eskjær Jensen, Armin Saalmüller, Nadine Wenzel, Constança Figueiredo · 发表于：Frontiers in Immunology · 年份：2024 · DOI：10.3389/fimmu.2024.1360022 · 被引用次数：6 · 研究领域：Xenotransplantation and immune response、Animal Disease Management and Epidemiology、T-cell and B-cell Immunology

Worldwide, pigs represent economically important farm animals, also representing a preferred preclinical large animal model for biomedical studies. The need for swine leukocyte antigen (SLA) typing is increasing with the expanded use of pigs in translational research, infection studies, and for veterinary vaccine design. Göttingen Minipigs (GMP) attract increasing attention as valuable model for pharmacological studies and transplantation research. This study represents a first-time assessment of the SLA gene diversity in Göttingen Minipigs in combination with a comparative metadata analysis with commercial pig lines. As Göttingen Minipigs could harbor private as well as potential novel SLA allele combinations, future research projects would benefit from the characterization of their SLA background. In 209 Göttingen Minipigs, SLA class I ( SLA-1 , SLA-2 , SLA-3 ) and class II ( DRB1 , DQB1 , DQA ) genes were characterized by PCR-based low-resolution (Lr) haplotyping. Criteria and nomenclature used for SLA haplotyping were proposed by the ISAG/IUIS-VIC SLA Nomenclature Committee. Haplotypes were assigned based on the comparison with already known breed or farm-specific allele group combinations. In total, 14 SLA class I and five SLA class II haplotypes were identified in the studied cohort, to manifest in 26 SLA class I but only seven SLA class II genotypes. The most common SLA class I haplotypes Lr-24.0 (SLA-1*15XX or Blank-SLA-3*04:04-SLA-2*06:01~02) and Lr-GMP-3.0 (SLA-1*16...