Redox‐Regulated Synthetic Channels: Enabling Reversible Ion Transport by Modulating the Ion‐Permeation Pathway

作者：Linlin Shi, Wen Zhao, Zhihui Jiu, Jingjing Guo, Qiuhui Zhu, Yonghui Sun, Bo Zhu, Junbiao Chang, Pengyang Xin · 发表于：Angewandte Chemie International Edition · 年份：2024 · DOI：10.1002/anie.202403667 · 被引用次数：30 · 研究领域：Ion channel regulation and function、Electrochemical Analysis and Applications、Molecular Sensors and Ion Detection

Natural redox-regulated channel proteins often utilize disulfide bonds as redox sensors for adaptive regulation of channel conformations in response to diverse physiological environments. In this study, we developed novel synthetic ion channels capable of reversibly switching their ion-transport capabilities by incorporating multiple disulfide bonds into artificial systems. X-ray structural analysis and electrophysiological experiments demonstrated that these disulfide-bridged molecules possess well-defined tubular cavities and can be efficiently inserted into lipid bilayers to form artificial ion channels. More importantly, the disulfide bonds in these molecules serve as redox-tunable switches to regulate the formation and disruption of ion-permeation pathways, thereby achieving a transition in the transmembrane transport process between the ON and OFF states.