Hydrogen peroxide sulfenylates and inhibits the photorespiratory enzyme PGLP1 to modulate plant thermotolerance

作者：Zheng-Wei Fu, Feng Ding, Binglei Zhang, Wen‐Cheng Liu, Zi-Hong Huang, Shihang Fan, Yu-Rui Feng, Ying‐Tang Lu, Wei Hua · 发表于：Plant Communications · 年份：2024 · DOI：10.1016/j.xplc.2024.100852 · 被引用次数：19 · 研究领域：Plant Stress Responses and Tolerance、Plant responses to elevated CO2、Light effects on plants

Climate change is resulting in more frequent and rapidly changing temperatures at both extremes that severely affect the growth and production of plants, particularly crops. Oxidative stress caused by high temperatures is one of the most damaging factors for plants. However, how H2O2 modulates plant thermotolerance is largely unknown. In addition, how photorespiration essential for C3 species is regulated remains enigmatic. Here, we report that heat stress promotes H2O2 accumulation in chloroplasts, H2O2 stimulates the sulfenylation of chloroplast-localized photorespiratory enzyme 2-phosphoglycolate phosphatase 1 (PGLP1) at cysteine 86 and inhibits its activity to promote the toxic metabolite 2-phosphoglycolate (2PG) accumulation. We also demonstrate that PGLP1 functions positively in plant thermotolerance, as PGLP1 antisense lines were more sensitive and PGLP1 overexpression plants showed higher tolerance to heat stress than the wild type. Together, our study illustrates heat-induced H2O2 in chloroplasts sulfenylates and inhibits PGLP1 to modulate plant thermotolerance. Furthermore, targeting CATALASE2 (CAT2) to chloroplasts largely prevented the heat-induced over-accumulation of H2O2 and sulfenylation of PGLP1, thus conferring thermotolerance without penalizing plant growth, revealing that heat-induced H2O2 in chloroplasts is important for heat-caused plant damage.