Multi-omics reveal mechanisms of high enteral starch diet mediated colonic dysbiosis via microbiome-host interactions in young ruminant

作者：Chunjia Jin, Shengru Wu, Ziqi Liang, Jun Zhang, Xinjian Lei, Hanxun Bai, Gaofeng Liang, Xiaodong Su, Xiaodong Chen, Peiyue Wang, Yue Wang, Leluo Guan, Junhu Yao · 发表于：Microbiome · 年份：2024 · DOI：10.1186/s40168-024-01760-w · 被引用次数：43 · 研究领域：Ruminant Nutrition and Digestive Physiology、Effects of Environmental Stressors on Livestock、Animal health and immunology

Abstract Background Although rumen development is crucial, hindgut undertakes a significant role in young ruminants’ physiological development. High-starch diet is usually used to accelerate rumen development for young ruminants, but always leading to the enteral starch overload and hindgut dysbiosis. However, the mechanism behind remains unclear. The combination of colonic transcriptome, colonic luminal metabolome, and metagenome together with histological analysis was conducted using a goat model, with the aim to identify the potential molecular mechanisms behind the disrupted hindgut homeostasis by overload starch in young ruminants. Result Compared with low enteral starch diet (LES), high enteral starch diet (HES)-fed goats had significantly higher colonic pathology scores, and serum diamine oxidase activity, and meanwhile significantly decreased colonic mucosal Mucin-2 (MUC2) protein expression and fecal scores, evidencing the HES-triggered colonic systemic inflammation. The bacterial taxa Prevotella sp. P4-67 , Prevotella sp. PINT , and Bacteroides sp. CAG:927 , together with fungal taxa Fusarium vanettenii , Neocallimastix californiae , Fusarium sp. AF-8 , Hypoxylon sp. EC38 , and Fusarium pseudograminearum , and the involved microbial immune pathways including the “T cell receptor signaling pathway” were higher in the colon of HES goats. The integrated metagenome and host transcriptome analysis revealed that these taxa were associated with enhanced pathogenic ability,...