Ameliorative effect and mechanism of ursodeoxycholic acid on hydrogen peroxide-induced hepatocyte injury

作者：Xueqin Wang, Guangxi Liang, Yang Zhou, B. Ni, Xiangyu Zhou · 发表于：Scientific Reports · 年份：2024 · DOI：10.1038/s41598-024-55043-3 · 被引用次数：15 · 研究领域：Eicosanoids and Hypertension Pharmacology、Liver Disease Diagnosis and Treatment、Drug-Induced Hepatotoxicity and Protection

Abstract To assess the ameliorative effect of ursodeoxycholic acid (UDCA) on hydrogen peroxide (H 2 O 2 )-induced hepatocyte injury. In our in vivo experiments, we modelled hyperlipidemia in ApoE −/− mice subjected to a 3-month high-fat diet and found that HE staining of the liver showed severe liver injury and excessive H 2 O 2 was detected in the serum. We modelled oxidative stress injury in L02 cells by H 2 O 2 in vitro and analyzed the levels of reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD) and related genes. UDCA significantly improved the level of oxidative stress in H 2 O 2 -injured L02 cells (P < 0.05). In addition, UDCA improved the transcription levels of inflammation and oxidative stress-related genes (P < 0.05), showing anti-inflammatory and anti-oxidative stress effects. UDCA has a protective effect on H 2 O 2 -damaged L02 cells, which lays a theoretical foundation for its application development.