Bacterial inoculants and enzymes based silage cocktails boost the ensiling quality of biomasses from reed, corn and rice straw

作者：Evan Y. Liu, Shuiping Wang, Shibo Wang, Nazir Ahmad Khan, Xiaoling Zhou, Shaoxun Tang, Chuanshe Zhou, Zhiliang Tan, Yong Liu · 发表于：Chemical and Biological Technologies in Agriculture · 年份：2024 · DOI：10.1186/s40538-024-00549-1 · 被引用次数：19 · 研究领域：Ruminant Nutrition and Digestive Physiology、Animal Nutrition and Physiology、Phytase and its Applications

Abstract This study investigated the effects of bacterial inoculants and enzyme-based silage cocktails on the dynamics of fermentation, microbiome, and nutritional value of silages produced from low-quality biomasses of reed, rice, and corn straw. A 90-day ensiling trial was performed using five distinct combinations of six basal bacterial species ( Lactobacillus plantarum , Lactobacillus buchneri , Pediococcus pentosaceus , Aspergillus niger , Bacillus subtilis , and Candida utilis ) and three basal enzymes (xylanase, β-mannanase, and glucanase). Each type of biomass was ensiled with six different treatments, including the Control treatment without an ensiling agent, the basal silage cocktail treatment (Mesa), and Mesa with a double dose of A. niger (MesaA), B. subtilis (MesaB), C. utilis (MesaC) and glucanase (MesaG). The “Mesa” contained (per kg silage), 1.0 × 10 6 CFU of L. plantarum , 1.4 × 10 7 CFU L. buchneri , 3.0 × 10 5 CFU P. pentosaceus , 8.0 × 10 8 CFU A. niger , 1.6 × 10 6 CFU B. subtilis and 1.0 × 10 9 CFU C. utilis , three enzymes (5.0 × 10 4 U xylanase, 2.5 × 10 3 U β-mannanase, and 1.0 × 10 4 U glucanase), and 20 mL molasses. Addition of the silage cocktails significantly improved the fermentation and nutritional quality of the reed, corn, and rice straw silages. Notably, the silage cocktails increased ( P < 0.01) the contents of crude protein (CP), ether extract (EE), gross energy (GE), lactic acid (LA), ratio of LA to total acids and ensiling comprehensi...