Target-activated multicolor fluorescent dyes for 3D imaging of plasma membranes and tracking of apoptosis

作者：Junjun Pan, Xin Peng, Chuangye Yao, Jiaqi Zuo, Tingting Lei, Hui Feng, Kewei Zhang, Engao Zhu, Zhaosheng Qian · 发表于：Journal of Materials Chemistry B · 年份：2024 · DOI：10.1039/d3tb02601a · 被引用次数：8 · 研究领域：Molecular Sensors and Ion Detection、Luminescence and Fluorescent Materials、Lipid Membrane Structure and Behavior

Real-time tracking of dynamic changes in the three-dimensional morphology of the cell plasma membrane is of great importance for a deeper understanding of physiological processes related to the cell plasma membrane. However, there is a lack of imaging dyes that can specifically be used for a long term labelling of plasma membranes, especially for plant cells. Here, we have used molecular engineering strategies to develop a series of target-activated multicolour fluorescent dyes that can be used for long-term and three-dimensional imaging of plant cell plasma membranes. By combining different electron acceptors and donors, four molecular backbones with different emission colours from green to NIR have been obtained. In the designed styrene-based dyes, referred to as the SD dyes, several functional groups were introduced into the backbones to achieve the properties of target-activated fluorescence, rapid and wash-free staining, high plasma membrane targeting ability and long-term imaging function. Using onion epidermal cells as a platform, these dye molecules can provide high-quality imaging of the plasma membrane for up to 6 hours, providing a powerful tool for long-term monitoring of plasma membrane-related biological events. Calcium-mediated apoptosis of plant cells has been tracked for the first time by monitoring the morphological changes of the plasma membrane in real time using SD dyes. These dyes also exhibit excellent 3D imaging performance of the plasma membrane and w...