Response surface methodology for aqueous two-phase system extraction: An unprecedented approach for the specific flavonoid-rich extraction of Houttuynia cordata Thunb. leaves towards acne treatment

作者：Hien Minh Nguyen, Lan Thi Nguyen, Nguyen Thien Han Le, Trong Nghia Ngoc Chau, Yen Nhi Thi Nguyen, Tan Ha, Phuoc Thuan Tran Nguyen, Thien Bao Chu, Chi Hieu Tran, Minh Tri Le · 发表于：Heliyon · 年份：2024 · DOI：10.1016/j.heliyon.2024.e25245 · 被引用次数：9 · 研究领域：Nephrotoxicity and Medicinal Plants、Chemical and Physical Properties in Aqueous Solutions、Heavy Metals in Plants

Background Houttuynia cordata Thunb. has long been widely used as a daily vegetable and traditional medicine. The flavonoid component of H. cordata has plenty of pharmacological effects, such as antibacterial, anti-inflammatory, and antioxidant. In this study, we applied the aqueous two-phase system (ATPS) combined with ultrasonic extraction for extracting H. cordata leaves. Methods We optimized the extraction process to improve the extraction efficiency of the two flavonoids, hyperin and quercitrin, by Surface Method Response - Central Composite Design (RSM-CCD). Next, we investigated the antibacterial ability of H. cordata ATPS extract from optimal conditions against two bacterial strains, Cutibacterium acnes and Staphylococcus epidermidis . Results The results showed that using 10% (NH 4 ) 2 SO 4 and 35% ethanol for ATPS extraction resulted in the highest hyperin and quercitrin contents. From the RSM-CCD results, the optimal extraction conditions were determined to be ultrasonic extraction at 50 °C for 30 min, giving results consistent with the predicted model and obtaining hyperin and quercitrin contents at 1.5681 ± 0.0114 and 4.6225 ± 0.0327 mg/g, respectively. Furthermore, ATPS extract has excellent antibacterial activity with a minimum inhibitory concentration (MIC) value of 250 μg/mL on both C. acnes and S. epidermidis . This MIC is significantly lower than the H. cordata ultrasound-assisted (UA) extract, with MICs of 1500.00 and 156.25 μg/mL on C. acnes and S. epider...