NIR-enhanced Pt single atom/g-C3N4 nanozymes as SOD/CAT mimics to rescue ATP energy crisis by regulating oxidative phosphorylation pathway for delaying osteoarthritis progression

作者：Jianhui Xiang, Xin Yang, Manli Tan, Jianfeng Guo, Yuting Ye, Jiejia Deng, Zhangrui Huang, Hanjie Wang, Wei Su, Jian‐Wen Cheng, Li Zheng, Sijia Liu, Jingping Zhong, Jinmin Zhao · 发表于：Bioactive Materials · 年份：2024 · DOI：10.1016/j.bioactmat.2024.02.018 · 被引用次数：66 · 研究领域：Advanced Nanomaterials in Catalysis、Nanoplatforms for cancer theranostics、Advanced biosensing and bioanalysis techniques

Osteoarthritis (OA) progresses due to the excessive generation of reactive oxygen and nitrogen species (ROS/RNS) and abnormal ATP energy metabolism related to the oxidative phosphorylation pathway in the mitochondria. Highly active single-atom nanozymes (SAzymes) can help regulate the redox balance and have shown their potential in the treatment of inflammatory diseases. In this study, we innovatively utilised ligand-mediated strategies to chelate Pt4+ with modified g-C3N4 by π–π interaction to prepare g–C3N4–loaded Pt single-atom (Pt SA/C3N4) nanozymes that serve as superoxide dismutase (SOD)/catalase (CAT) mimics to scavenge ROS/RNS and regulate mitochondrial ATP production, ultimately delaying the progression of OA. Pt SA/C3N4 exhibited a high loading of Pt single atoms (2.45 wt%), with an excellent photothermal conversion efficiency (54.71%), resulting in tunable catalytic activities under near-infrared light (NIR) irradiation. Interestingly, the Pt–N6 active centres in Pt SA/C3N4 formed electron capture sites for electron holes, in which g-C3N4 regulated the d-band centre of Pt, and the N-rich sites transferred electrons to Pt, leading to the enhanced adsorption of free radicals and thus higher SOD- and CAT-like activities compared with pure g-C3N4 and g–C3N4–loaded Pt nanoparticles (Pt NPs/C3N4). Based on the use of H2O2-induced chondrocytes to simulate ROS-injured cartilage in vitro and an OA joint model in vivo, the results showed that Pt SA/C3N4 could reduce oxidativ...