Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle

作者：Jinzhu Meng, Qiuye Li, Lilin Xiao, Weichen Liu, Zhengjie Gao, Lin Gong, Xianyong Lan, Shuilian Wang · 发表于：Frontiers in Endocrinology · 年份：2024 · DOI：10.3389/fendo.2023.1275022 · 被引用次数：4 · 研究领域：Reproductive Physiology in Livestock、Genetic and phenotypic traits in livestock、Cancer-related molecular mechanisms research

The objective of the present study was to investigate the potential role of immunization against INH on follicular development, serum reproductive hormone (FSH, E 2 , and P 4 ) concentrations, and reproductive performance in beef cattle. A total of 196 non-lactating female beef cattle (4-5 years old) with identical calving records (3 records) were immunized with 0.5, 1.0, 1.5, or 2.0 mg [(T1, n = 58), (T2, n = 46), (T3, n = 42) and (T4, n = 36), respectively] of the pcISI plasmid. The control (C) group (n = 14) was immunized with 1.0 mL 0.9% saline. At 21d after primary immunization, all beef cattle were boosted with half of the primary immunization dose. On day 10 after primary immunization, the beef cattle immunized with INH DNA vaccine evidently induced anti-INH antibody except for the T1 group. The T3 group had the greatest P/N value peak among all the groups. The anti-INH antibody positive rates in T2, T3 and T4 groups were significantly higher than that in C and T1 groups. RIA results indicated that serum FSH concentration in T2 group increased markedly on day 45 after booster immunization; the E 2 amount in T3 group was significantly increased on day 10 after primary immunization, and the levels of E 2 also improved in T2 and T3 groups after booster immunization; the P 4 concentration in T2 group was significantly improved on day 21 after primary immunization. Ultrasonography results revealed that the follicles with different diameter sizes were increased, meanwhile, t...