The application of silver nanoparticles green-formulated by Origanum majorana leaf extract as a hydrazine sensor and treatment of human breast cancer by following the P53 and STAT3 signaling pathways

作者：Chao Qian, Xueli Dong, Shaik Althaf Hussain, Lei Wang, Guoliang Zhao · 发表于：Journal of Science Advanced Materials and Devices · 年份：2024 · DOI：10.1016/j.jsamd.2024.100691 · 被引用次数：12 · 研究领域：Nanoparticles: synthesis and applications、Nanoparticle-Based Drug Delivery、Nanoplatforms for cancer theranostics

Silver nanoparticles, because of their large absorption surface and small size, are considered to be intelligent magnetic particles. The advancements in nanotechnology have revolutionized cancer treatment, with silver nanoparticles playing a crucial role in this field. In a recent experiment, the effects of Ag nanoparticles formulated from Origanum majorana on breast cancer cells were investigated. It was found that these nanoparticles induce apoptosis through the signal transducer and activator of transcription 3 and P53 signaling pathways. The nanoparticles characterization was conducted using FE-SEM, XRD, and UV–Vis techniques. Furthermore, the Ag nanoparticles exhibited significant antioxidant activity by preventing 50% of DPPH at 183 μg/mL. The MTT assay revealed the anti-breast carcinoma properties of silver NPs on MCF-7, T-47D, and SkBr3 cells. The outcomes demonstrated that as the nanoparticles concentration increased, the cancer cells survival percentage reduced for 3 days. The most effective anticancer effect was observed at 1000 μg/ml. MTT findings indicate that a concentration of nanoparticles at IC50 = 97, 186, and 180 μg/ml effectively targets 50% of MCF-7, T-47D, and SkBr3 breast carcinoma cells. The presence of silver NPs induces cell apoptosis, which is with the Bax markers regulation and the pro-apoptotic cleaved caspase-8 upregulation, while the anti-apoptotic Bcl-2 marker is downregulated. Besides, silver NPs inhibit the formation of colonies. Molecular pa...