The genome of Stephania japonica provides insights into the biosynthesis of cepharanthine

作者：Zhuo Liu, Shaoqin Shen, Yujie Wang, Sun Shuqi, Tong Yu, Yanhong Fu, Rong Zhou, Chunjin Li, Rui Cao, Yanshu Zhang, Nan Li, Liangdan Sun, Xiaoming Song · 发表于：Cell Reports · 年份：2024 · DOI：10.1016/j.celrep.2024.113832 · 被引用次数：10 · 研究领域：Genomics and Phylogenetic Studies、Genetic diversity and population structure、Plant Pathogenic Bacteria Studies

Stephania japonica is an early-diverging eudicotyledon plant with high levels of cepharanthine, proven to be effective in curing coronavirus infections. Here, we report a high-quality S. japonica genome. The genome size is 688.52 Mb, and 97.37% sequences anchor to 11 chromosomes. The genome comprises 67.46% repetitive sequences and 21,036 genes. It is closely related to two Ranunculaceae species, which diverged from their common ancestor 55.90-71.02 million years ago (Mya) with a whole-genome duplication 85.59-96.75 Mya. We further reconstruct ancestral karyotype of Ranunculales. Several cepharanthine biosynthesis genes are identified and verified by western blot. Two genes (Sja03G0243 and Sja03G0241) exhibit catalytic activity as shown by liquid chromatography-mass spectrometry. Then, cepharanthine biosynthesis genes, transcription factors, and CYP450 family genes are used to construct a comprehensive network. Finally, we construct an early-diverging eudicotyledonous genome resources (EEGR) database. As the first genome of the Menispermaceae family to be released, this study provides rich resources for genomic studies.