Photosensitive Hydrogels Encapsulating DPSCs and AgNPs for Dental Pulp Regeneration

作者：Yan He, Yanni Zhang, Fengting Hu, Min Chen, Ben Wang, Yejian Li, Haichao Xu, Na Dong, Chen Zhang, Yunfan Hu, Zhiqiang Lin, Youjian Peng, Qingsong Ye, Lihua Luo · 发表于：International Dental Journal · 年份：2024 · DOI：10.1016/j.identj.2024.01.017 · 被引用次数：13 · 研究领域：Endodontics and Root Canal Treatments、Mesenchymal stem cell research、Tissue Engineering and Regenerative Medicine

OBJECTIVE: Pulp regeneration with bioactive dentin-pulp complex has been a research hotspot in recent years. Stem cell therapy provided an interest strategy to regenerate the dental-pulp complex. Hence, this study aimed to evaluate the effects of photosensitive gelatin methacrylate (GelMA) hydrogel encapsulating dental pulp stem cells (DPSCs) and silver nanoparticles (AgNPs) for dental pulp regeneration in vitro. METHODS: First, the AgNPs@GelMA hydrogels were prepared by lithium phenyl-2,4,6-trimethyl-benzoyl phosphinate (LAP) initiation via blue-light emitting diode light. The physical and chemical properties of AgNPs@GelMA hydrogels were comprehensively analysed via scanning electron microscopy (SEM), and mechanical characterisation, such as swelling ability, degradation properties, and AgNP release profile. Then, AgNPs@GelMA hydrogels encapsulated DPSCs were used to establish an AgNPs@GelMA biomimetic complex, further analysing its biocompatibility, antibacterial properties, and angiogenic capacity in vitro. RESULTS: The results indicated that GelMA hydrogels demontrated optimal characteristics with a monomer:LAP ratio of 16:1. The physico-chemical properties of AgNPs@GelMA hydrogels did not change significantly after loading with AgNPs. There was no significant difference in AgNP release rate amongst different concentrations of AgNPs@GelMA hydrogels. Fifty to 200 μg/mL AgNPs@GelMA hydrogels could disperse E faecalis biofilm and reduce its metabolic activity . Furthermore,...