Transcriptome analysis reveals the potential mechanism of altering viability, yield, and isoquinoline alkaloids in Coptis chinensis through Cunninghamia lanceolata understory cultivation

作者：Yuanyuan Duan, Jingmao You, Jintao Wang, Tao Tang, Xiaoliang Guo, Fanfan Wang, Xiaoyue Wang, Sen Mu, Qingfang Wang, Xiaofeng Niu, Jie Guo · 发表于：Chemical and Biological Technologies in Agriculture · 年份：2024 · DOI：10.1186/s40538-024-00548-2 · 被引用次数：8 · 研究领域：Ginseng Biological Effects and Applications、Berberine and alkaloids research、Plant Stress Responses and Tolerance

Abstract The dried rhizomes of Coptis chinensis hold significance in Chinese medicine. Monocropping C. chinensis under the shade of a manmade scaffold, the primary planting mode, poses a threat to the ecological balance. In recent years, the practice of C. chinensis–Cunninghamia lanceolata understory cultivation has gained prevalence in southwest China. However, there is no evidence to suggest that understory cultivation enhances the viability, yield, or isoquinoline alkaloid content of C. chinensis . This study examined the physiological properties, yield indicators, and isoquinoline alkaloid content to investigate variations in C. chinensis in response to understory cultivation. Transcriptome analysis was conducted to elucidate potential mechanisms driving these alterations. The results indicate that understory cultivation significantly enhances the viability, yield, and levels of epiberberine, palmatine, and berberine in C. chinensis while reducing coptisine content. Transcriptomic analyses identified 2062 upregulated and 1853 downregulated genes in the understory cultivation system. Pathways such as “phenylpropanoid biosynthesis,” “zeatin biosynthesis,” “photosynthesis,” “tyrosine metabolism,” “isoquinoline alkaloid biosynthesis,” and “starch and sucrose metabolism” exhibited significant enrichment of differentially expressed genes (DEGs). DEGs involved in these pathways were thoroughly analyzed. INV , BGL-2 , BGL-4 , SPS-2 , AMY-3 , Psb B , Psb R , Psb S , Psa D , Psa E ...