GhBRX.1, GhBRX.2, and GhBRX4.3 improve resistance to salt and cold stress in upland cotton

作者：Wei Wei, Jisheng Ju, Xueli Zhang, Xueli Zhang, Pingjie Ling, Jin Luo, Ying Li, Wenjuan Xu, Junji Su, Xianliang Zhang, Xianliang Zhang, Caixiang Wang · 发表于：Frontiers in Plant Science · 年份：2024 · DOI：10.3389/fpls.2024.1353365 · 被引用次数：10 · 研究领域：Research in Cotton Cultivation、Plant Molecular Biology Research、Plant Virus Research Studies

Introduction Abiotic stress during growth readily reduces cotton crop yield. The different survival tactics of plants include the activation of numerous stress response genes, such as BREVIS RADIX ( BRX ). Methods In this study, the BRX gene family of upland cotton was identified and analyzed by bioinformatics method, three salt-tolerant and cold-resistant GhBRX genes were screened. The expression of GhBRX.1 , GhBRX.2 and GhBRXL4.3 in upland cotton was silenced by virus-induced gene silencing (VIGS) technique. The physiological and biochemical indexes of plants and the expression of related stress-response genes were detected before and after gene silencing. The effects of GhBRX.1 , GhBRX.2 and GhBRXL4.3 on salt and cold resistance of upland cotton were further verified. Results and discussion We discovered 12, 6, and 6 BRX genes in Gossypium hirsutum , Gossypium raimondii and Gossypium arboreum , respectively. Chromosomal localization indicated that the retention and loss of GhBRX genes on homologous chromosomes did not have a clear preference for the subgenomes. Collinearity analysis suggested that segmental duplications were the main force for BRX gene amplification. The upland cotton genes GhBRX.1 , GhBRX.2 and GhBRXL4.3 are highly expressed in roots, and GhBRXL4.3 is also strongly expressed in the pistil. Transcriptome data and qRT‒PCR validation showed that abiotic stress strongly induced GhBRX.1 , GhBRX.2 and GhBRXL4.3 . Under salt stress and low-temperature stress con...