Astragalus polysaccharides attenuate rat aortic endothelial senescence via regulation of the SIRT-1/p53 signaling pathway

作者：Xinyu Miao, Lingjun Rong, Bo Fu, Shaoyuan Cui, Zhaoyan Gu, Fan Hu, Yanhui Lu, Shuangtong Yan, Banruo Sun, Wen‐Li Jiang, Yuting Zhang, Yanping Gong, Chunlin Li · 发表于：BMC Complementary Medicine and Therapies · 年份：2024 · DOI：10.1186/s12906-024-04387-4 · 被引用次数：17 · 研究领域：Telomeres, Telomerase, and Senescence、Traditional Chinese Medicine Analysis、Antioxidants, Aging, Portulaca oleracea

Abstract Background Astragalus polysaccharides (APS) have been verified to have antioxidative and antiaging activities in the mouse liver and brain. However, the effect of APS on aortic endothelial senescence in old rats and its underlying mechanism are currently unclear. Here, we aimed to elucidate the effects of APS on rat aortic endothelial oxidative stress and senescence in vitro and in vivo and investigate the potential molecular targets. Methods Twenty-month-old natural aging male rats were treated with APS (200 mg/kg, 400 mg/kg, 800 mg/kg daily) for 3 months. Serum parameters were tested using corresponding assay kits. Aortic morphology was observed by staining with hematoxylin and eosin (H&E) and Verhoeff Van Gieson (VVG). Aging-related protein levels were evaluated using immunofluorescence and western blot analysis. Primary rat aortic endothelial cells (RAECs) were isolated by tissue explant method. RAEC mitochondrial function was evaluated by the mitochondrial membrane potential (MMP) measured with the fluorescent lipophilic cationic dye JC‑1. Intracellular total antioxidant capacity (T-AOC) was detected by a commercial kit. Cellular senescence was assessed using senescence-associated-β-galactosidase (SA-β-Gal) staining. Results Treatment of APS for three months was found to lessen aortic wall thickness, renovate vascular elastic tissue, improve vascular endothelial function, and reduce oxidative stress levels in 20-month-old rats. Primary mechanism analysis sho...