Efficient haploid induction via egg cell expression of dandelion PARTHENOGENESIS in foxtail millet (Setaria italica)

作者：Yong Huang, Yuqing Liang, Yingying Xie, Yuchun Rao, Jie Xiong, Chaolei Liu, Chun Wang, Xingchun Wang, Qian Qian, Kejian Wang · 发表于：Plant Biotechnology Journal · 年份：2024 · DOI：10.1111/pbi.14302 · 被引用次数：23 · 研究领域：Chromosomal and Genetic Variations、Plant Taxonomy and Phylogenetics、Plant nutrient uptake and metabolism

Foxtail millet (Setaria italica) is one of the most significant multigrain crops in arid and semi-arid regions worldwide. Given its relatively small genome size, strong environmental adaptability, high C4 light efficiency and remarkable nutritional value, foxtail millet is emerging as a rapidly developing C4 model crop for research on drought resistance, nutrition efficiency, and photosynthesis (Peng and Zhang, 2021). Doubled haploid (DH) technology has the potential to revolutionize crop breeding efficiency by rapidly fixing recombinant haplotypes within just two generations compared to traditional breeding methods (Jacquier et al., 2020). However, the limited research on haploid induction systems in foxtail millet hampers the widespread application of DH technology in this crop. Here, we report the successful generation of haploid plants through seed by egg cell expression of dandelion PARTHENOGENESIS (PAR), providing the possibility for further advancements in DH technology utilization in foxtail millet breeding programs. Dandelion (Taraxacum officinale) is a well-known apomictic plant that predominantly reproduces through parthenogenesis. The recent discovery of the PAR gene has provided insights into the genetic mechanism underlying parthenogenesis in dandelions. This gene encodes a K2-2 zinc finger domain protein with an EAR (ethylene-responsive element-binding factor-associated amphiphilic repression) motif, exerting a dominant effect on apomixis in dandelion. Furtherm...