Influence of weeding methods on rhizosphere soil and root endophytic microbial communities in tea plants

作者：Yuxiao Yan, Conglian Wang, Renyuan Wan, Shuang Li, Yanfen Yang, Caiyou Lv, Yongmei Li, Yang Guang-rong · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1334711 · 被引用次数：7 · 研究领域：Plant-Microbe Interactions and Immunity、Allelopathy and phytotoxic interactions、Plant Disease Resistance and Genetics

Introduction Polyethylene mulch is a kind of inorganic mulch widely used in agriculture. The effects of plastic mulch debris on the structure of plant soil and root growth have been fully studied, but their effects on endophytic microbial communities have not been explored to a large extent. Methods In this study, High-throughput sequencing of bacterial 16S rRNA genes and fungal ITS region sequences were used to analyze microbial community structure and composition in rhizosphere soil and root endophytic of tea plant under three different weeding methods: polyethylene mulching, hand weeding and no weeding (CK). Results The results showed that the weeding methods had no significant effect on the rhizosphere and root endophytic microbial abundance, but the rhizosphere bacterial structure covered by polyethylene mulch was significantly different than hand weeding and CK. The rhizosphere fungal diversity was also significantly higher than the other two analyzed treatments. The community abundance of rhizosphere microorganisms Acidobacteria, Candidatus Rokubacteria and Aspergillus covered by polyethylene mulch decreased significantly, whereas Bradyrhizobium , Solirubrobacterales and Alphaproteobacteria increased significantly. The abundance of bacteria Ktedonobacter , Reticulibacter , Ktedonosporobacter and Dictyobacter communities covered by polyethylene mulch was significantly changed, and the abundance of Fusarium and Nitrobacteraceae was significantly increased. Rhizosphere do...