Porous Se@SiO2 nanospheres alleviate diabetic retinopathy by inhibiting excess lipid peroxidation and inflammation

作者：Tian Niu, Xin Shi, Xijian Liu, Haiyan Wang, Kun Liu, Yupeng Xu · 发表于：Molecular Medicine · 年份：2024 · DOI：10.1186/s10020-024-00785-z · 被引用次数：26 · 研究领域：Selenium in Biological Systems、Advanced Nanomaterials in Catalysis、Retinal Diseases and Treatments

Abstract Background Lipid peroxidation is a characteristic metabolic manifestation of diabetic retinopathy (DR) that causes inflammation, eventually leading to severe retinal vascular abnormalities. Selenium (Se) can directly or indirectly scavenge intracellular free radicals. Due to the narrow distinction between Se’s effective and toxic doses, porous Se@SiO2 nanospheres have been developed to control the release of Se. They exert strong antioxidant and anti-inflammatory effects. Methods The effect of anti-lipid peroxidation and anti-inflammatory effects of porous Se@SiO 2 nanospheres on diabetic mice were assessed by detecting the level of Malondialdehyde (MDA), glutathione peroxidase 4 (GPX4), decreased reduced/oxidized glutathione (GSH/GSSG) ratio, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and interleukin (IL) -1β of the retina. To further examine the protective effect of porous Se@SiO 2 nanospheres on the retinal vasculopathy of diabetic mice, retinal acellular capillary, the expression of tight junction proteins, and blood–retinal barrier destruction was observed. Finally, we validated the GPX4 as the target of porous Se@SiO 2 nanospheres via decreased expression of GPX4 and detected the level of MDA, GSH/GSSG, TNF-α, IFN-γ, IL -1β, wound healing assay, and tube formation in high glucose (HG) cultured Human retinal microvascular endothelial cells (HRMECs). Results The porous Se@SiO 2 nanospheres reduced the level of MDA, TNF-α, IFN-γ, and IL -1β, while increasi...