The neuroprotective effects of Rehmanniae Radix Praeparata exerts via regulating SKN-1 mediated antioxidant system in Caenorhabditis elegans and activating Nrf2-ARE pathway in vitro

作者：Renjuan Hao, Ying-Chao Hu, Yu-Yan Wei, Xiude Qin, Yun-Wei Lu · 发表于：Journal of Functional Foods · 年份：2024 · DOI：10.1016/j.jff.2024.106040 · 被引用次数：9 · 研究领域：Medicinal Plants and Neuroprotection、Genetics, Aging, and Longevity in Model Organisms、Alzheimer's disease research and treatments

Alzheimer's disease (AD) is an age-related neurodegenerative disease, which manifests primarily as memory impairment accompanied by a gradual decline in cognitive function. Rehmanniae Radix Praeparata (RRP) is a traditional Chinese medicine herb, which is widely used clinically and has diverse pharmacological properties. However, the protective properties and the underlying mechanisms by which RRP counteracts oxidative stress to treat AD have not been elucidated. In the study, we explored the neuroprotective impact of RRP on mitigating oxidative stress damage using C. elegans and Aβ1-42-induced HT22 cells. In the study encompassing the use of HT22 cells, we detected cell viability, ROS level, MDA/T-AOC/CAT/SOD activity, HT22 cells apoptosis and Nrf2 translocation. Western blot analysis was used to examine changes in protein expression levels. In nematode experiments, we performed spawning assay, life-span assay, ROS level, MDA/T-AOC/CAT/SOD activity, chemotaxis assay, paralysis, serotonin sensitivity, Th S Staining, acridine orange assay, SKN-1 nuclear localization and the gst-4 expression. Pretreatment of RRP significantly reduced the generation of ROS and MDA in Aβ1-42-induced HT22 cells, and increase the T-AOC, CAT and total SOD activity. RRP mitigated the toxic effect and apoptosis induced by Aβ1-42 treatment, as evident by reduced cytochrome C release and decreased ratio of cleaved caspase-9/caspase-9, BAX/Bcl-2 and cleaved caspase-3/caspase-3. Additionally, RRP was foun...