Chromosome-level genome assembly and annotation of the yellow grouper, Epinephelus awoara

作者：Weiwei Zhang, Yang Yang, Sijie Hua, Qingxin Ruan, Duo Li, Le Wang, Xi Wang, Xin Wen, Xiaochun Liu, Zining Meng · 发表于：Scientific Data · 年份：2024 · DOI：10.1038/s41597-024-02989-8 · 被引用次数：9 · 研究领域：Genomics and Phylogenetic Studies、Aquaculture disease management and microbiota、Chromosomal and Genetic Variations

Epinephelus awoara, as known as yellow grouper, is a significant economic marine fish that has been bred artificially in China. However, the genetic structure and evolutionary history of yellow grouper remains largely unknown. Here, this work presents the high-quality chromosome-level genome assembly of yellow grouper using PacBio single molecule sequencing technique (SMRT) and High-through chromosome conformation capture (Hi-C) technologies. The 984.48 Mb chromosome-level genome of yellow grouper was assembled, with a contig N50 length of 39.77 Mb and scaffold N50 length of 41.39 Mb. Approximately 99.76% of assembled sequences were anchored into 24 pseudo-chromosomes with the assistance of Hi-C reads. Furthermore, approximately 41.17% of the genome was composed of repetitive elements. In total, 24,541 protein-coding genes were predicted, of which 22,509 (91.72%) genes were functionally annotated. The highly accurate, chromosome-level reference genome assembly and annotation are crucial to the understanding of population genetic structure, adaptive evolution and speciation of the yellow grouper.