A human serum albumin-indocyanine green complex offers improved tumor identification in fluorescence-guided surgery

作者：Yunlong Li, Chun Dai, Zhaolai Hua, Lin Xia, Yongbin Ding, Qiang Wang, Marie-Laure Matthey Gié, Michael Bouvet, Huiming Cai · 发表于：Translational Cancer Research · 年份：2024 · DOI：10.21037/tcr-23-2338 · 被引用次数：12 · 研究领域：Cancer Research and Treatments、Nanoplatforms for cancer theranostics、Photodynamic Therapy Research Studies

Background: Complete tumor removal is critical for achieving a good prognosis in patients but remains challenging for surgeons. Near-infrared fluorescence-guided surgery (NIRFGS) enables surgeons to accurately localize tumors in real time and facilitates accurate resection. Indocyanine green (ICG) has been approved by the U.S. Food and Drug Administration and the National Medical Products Administration for many years. Although the application of ICG has progressed for a variety of surgeries, there are inherent limitations to ICG, including poor water solubility and photostability, short blood half-life, and aggregation in blood, resulting in poor imaging performance. We found that mixing ICG with human serum albumin (HSA) preoperatively and then injecting it can improve the imaging performance. Methods: We prepared fluorescent probes by combining ICG with HSA and identified their optimal ratio via in vitro absorption measurement and emission spectrum characterization of ICG-HSA complex with different mixing ratios and concentration gradients. Subsequently, under the optimal ratio and clinical simulated concentration, we conducted dynamic change analysis of the fluorescence spectral properties after mixing. We then compared the uptake of ICG-HSA in vitro for two different cell types and the imaging performance of different molar ratios of ICG and HSA in mouse models. Results: Through in vitro absorption and emission spectrum characterization of ICG-HSA mixtures with different...