Cell surface architecture of the cultivated DPANN archaeon Nanobdella aerobiophila

作者：Shingo Kato, Yuhei O. Tahara, Yuki Nishimura, Katsuyuki Uematsu, Takahiro Arai, Daisuke Nakane, Ayaka Ihara, Takayuki Nishizaka, Wataru Iwasaki, Takashi Itoh, Makoto Miyata, Moriya Ohkuma · 发表于：Journal of Bacteriology · 年份：2024 · DOI：10.1128/jb.00351-23 · 被引用次数：8 · 研究领域：Bacteriophages and microbial interactions、Genomics and Phylogenetic Studies、RNA and protein synthesis mechanisms

ABSTRACT The DPANN archaeal clade includes obligately ectosymbiotic species. Their cell surfaces potentially play an important role in the symbiotic interaction between the ectosymbionts and their hosts. However, little is known about the mechanism of ectosymbiosis. Here, we show cell surface structures of the cultivated DPANN archaeon Nanobdella aerobiophila strain MJ1 T and its host Metallosphaera sedula strain MJ1HA, using a variety of electron microscopy techniques, i.e., negative-staining transmission electron microscopy, quick-freeze deep-etch TEM, and 3D electron tomography. The thickness, unit size, and lattice symmetry of the S-layer of strain MJ1 T were different from those of the host archaeon strain MJ1HA. Genomic and transcriptomic analyses highlighted the most highly expressed MJ1 T gene for a putative S-layer protein with multiple glycosylation sites and immunoglobulin-like folds, which has no sequence homology to known S-layer proteins. In addition, genes for putative pectin lyase- or lectin-like extracellular proteins, which are potentially involved in symbiotic interaction, were found in the MJ1T genome based on in silico 3D protein structure prediction. Live cell imaging at the optimum growth temperature of 65°C indicated that cell complexes of strains MJ1 T and MJ1HA were motile, but sole MJ1 T cells were not. Taken together, we propose a model of the symbiotic interaction and cell cycle of Nanobdella aerobiophila . IMPORTANCE DPANN archaea are widely dist...