Supernatant of platelet- Klebsiella pneumoniae coculture induces apoptosis-like death in Klebsiella pneumoniae

作者：Wenting Wang, Yaozhen Chen, Yutong Chen, Erxiong Liu, Jing Li, Ning An, Jinmei Xu, Shunli Gu, Xuan Dang, Jing Yi, Qunxing An, Xingbin Hu, Wen Yin · 发表于：Microbiology Spectrum · 年份：2024 · DOI：10.1128/spectrum.01279-23 · 被引用次数：3 · 研究领域：Antibiotic Resistance in Bacteria、Antibiotics Pharmacokinetics and Efficacy、Drug-Induced Adverse Reactions

ABSTRACT Multidrug-resistant Klebsiella pneumoniae strains, especially carbapenem-resistant K. pneumoniae , have become a rapidly emerging crisis worldwide, greatly limiting current therapeutic options and posing new challenges to infection management. Therefore, it is imperative to develop novel and effective biological agents for the treatment of multidrug-resistant K. pneumoniae infections. Platelets play an important role in the development of inflammation and immune responses. The main component responsible for platelet antibacterial activity lies in the supernatant stimulated by gram-positive bacteria. However, little research has been conducted on the interaction of gram-negative bacteria with platelets. Therefore, we aimed to explore the bacteriostatic effect of the supernatant derived from platelet- K. pneumoniae coculture and the mechanism underlying this effect to further assess the potential of platelet-bacterial coculture supernatant. We conducted this study on the gram-negative bacteria K. pneumoniae and CRKP and detected turbidity changes in K. pneumoniae and CRKP cultures when grown with platelet- K. pneumoniae coculture supernatant added to the culture medium. We found that platelet- K. pneumoniae coculture supernatant significantly inhibited the growth of K. pneumoniae and CRKP in vitro . Furthermore, transfusion of platelet- K. pneumoniae coculture supernatant alleviated the symptoms of K. pneumoniae and CRKP infection in a murine model. Additionally, we ob...