Endometrial transcriptome profiling of patients with recurrent implantation failure during hormone replacement therapy cycles

作者：Wen-bi Zhang, Jue Li, Qing Li, Xiang Lü, Junling Chen, Lu Li, Hua Chen, Wei Fu, Jiu-cheng Chen, Bingjie Lu, Han Wu, Xiaoxi Sun · 发表于：Frontiers in Endocrinology · 年份：2024 · DOI：10.3389/fendo.2023.1292723 · 被引用次数：9 · 研究领域：Reproductive System and Pregnancy、Endometriosis Research and Treatment、Ovarian function and disorders

Background: The molecular mechanisms underlying window of implantation (WOI) displacement in patients with recurrent implantation failure (RIF) remain unclear. This study aims to explore the transcriptomic signatures of endometrium with normal and displaced WOIs and to identify the causes of endometrial receptivity (ER) abnormalities and WOI displacement in RIF patients. Methods: In this study, 40 RIF patients were recruited and underwent personalized embryo transfer (pET) guided by the predicted results of endometrial receptivity diagnosis (ERD) model. Transcriptome analysis of endometrium from patients with clinical pregnancies after pET was performed to identify differentially expressed genes (DEGs) associated with WOI displacement. Gene expression data from HRT and natural cycle endometrium were compared to identify specific gene expression patterns of ER-related genes during WOI. Results: The ERD results indicated that 67.5% of RIF patients (27/40) were non-receptive in the conventional WOI (P+5) of the HRT cycle. The clinical pregnancy rate in RIF patients improved to 65% (26/40) after ERD-guided pET, indicating the effectiveness of transcriptome-based WOI prediction. Among the 26 patients with clinical pregnancy, the gene expression profiles of P+5 endometrium from advanced (n=6), normal (n=10) and delayed (n=10) WOI groups were significantly different from each other. Furthermore, 10 DEGs identified among P+5 endometrium of 3 groups were involved in immunomodulation, ...