Opah (Lampris megalopsis) genome and the genetic mechanisms underlying whole-body endothermy

作者：Jing Bo, Wenqi Lv, Ning Sun, Cheng Wang, Kun Wang, Pan Liu, Chenguang Feng, Liandong Yang, Shunping He · 年份：2024 · DOI：10.22541/au.170665353.30681873/v1 · 被引用次数：1 · 研究领域：Physiological and biochemical adaptations、Adipose Tissue and Metabolism、Neurobiology and Insect Physiology Research

Endothermy (production and retention of metabolic heat) enhances the physiological functions of mammals, birds and a few fish species. The opah, Lampris megalopsis (Lampridiformes), is the only known teleost with whole-body endothermy that confers physiological and ecological advantages in competition. The genetic mechanisms of opah whole-body endothermy are unclear. We sequenced the opah genome and assembled a high-resolution genome. Through analysis of positive selection, rapid evolution, and family expansion, we discovered several genes that likely contributed to the development of dark red pectoral muscles, uncoupling protein sarcolipin mediated heat production, retia mirabilia and adipose metabolism in opah. A functional experiment showed that the positively selected glycolysis enzyme LDHM exhibits higher catalytic activity in energy supply related pathways. The results illustrate possible molecular and genetic mechanisms involved in whole-body endothermy in fish.