Integrative analysis of metabolome and transcriptome provides new insights into functional components of Lilii Bulbus

作者：Wenjun Wei, Tao Guo, Wenguang Fan, Mengshan Ji, Yu Fu, Conglong Lian, Suiqing Chen, Wenjing Ma, Wenfang Ma, Shuying Feng · 发表于：Chinese Herbal Medicines · 年份：2024 · DOI：10.1016/j.chmed.2023.10.004 · 被引用次数：11 · 研究领域：Phytochemical Studies and Bioactivities、Phytochemical and Pharmacological Studies、Phytochemistry and biological activities of Ficus species

Lilium brownii var. viridulum (LB) and L. lancifolium (LL) are the main sources of medicinal lily (Lilii Bulbus, Baihe in Chinese) in China. However, the functional components of these two species responsible for the treatment efficacy are yet not clear. In order to explore the therapeutic material basis of Lilii Bulbus, we selected L. davidii var. willmottiae (LD) only used for food as the control group to analyze the differences between LD and the other two (LB and LL). Metabolome and transcriptome were carried out to investigate the differences of active components in LD vs LB and LD vs LL. Data of metabolome and transcriptome was analysed using various analysis methods, such as principal component analysis (PCA), hierarchical cluster analysis (HCH) analysis, and so on. Differentially expressed genes (DEGs) were enriched through KEGG and GO enrichment analysis, The PCA and HCH analysis of the metabolome indicated the metabolites were clearly separated and varied greatly in LL and LB contrasted with LD. There were 318 significantly differential metabolites (SDMs) in LD vs LB group and 298 SDMs in LD vs LL group. Compared with LD group, the significant up-regulation of steroidal saponins and steroidal alkaloids were detected both in LB and LL groups, especially in LB group. The HCH analysis of transcriptome indicated that there was significant difference in LB vs LD group, while the difference between LL and LD varied slightly. Additionally, 47 540 DEGs in LD vs LB group and...