Chitosan alleviates ovarian aging by enhancing macrophage phagocyte-mediated tissue homeostasis

作者：Hui‐Hui Shen, Xinyan Zhang, Nan Liu, Yangyang Zhang, Huihua Wu, Feng Xie, Wenjun Wang, Ming‐Qing Li · 发表于：Immunity & Ageing · 年份：2024 · DOI：10.1186/s12979-024-00412-9 · 被引用次数：22 · 研究领域：Reproductive Biology and Fertility、Ovarian cancer diagnosis and treatment、Ovarian function and disorders

Abstract Background Age-related changes in the ovarian microenvironment are linked to impaired fertility in women. Macrophages play important roles in ovarian tissue homeostasis and immune surveillance. However, the impact of aging on ovarian macrophage function and ovarian homeostasis remains poorly understood. Methods Senescence-associated beta-galactosidase staining, immunohistochemistry, and TUNEL staining were used to assess senescence and apoptosis, respectively. Flow cytometry was employed to evaluate mitochondrial membrane potential (MMP) and apoptosis in granulosa cells lines (KGN), and macrophages phagocytosis. After a 2-month treatment with low molecular weight Chitosan (LMWC), ovarian tissues from mice were collected for comprehensive analysis. Results Compared with the liver and uterus, the ovary displayed accelerated aging in an age-dependent manner, which was accompanied by elevated levels of inflammatory factors and apoptotic cells, and impaired macrophage phagocytic activity. The aged KGN cells exhibited elevated reactive oxygen species (ROS) and apoptotic levels alongside decreased MMP. H 2 O 2 -induced aging macrophages showed reduced phagocytosis function. Moreover, there were excessive aging macrophages with impaired phagocytosis in the follicular fluid of patients with diminished ovarian reserve (DOR). Notably, LMWC administration alleviated ovarian aging by enhancing macrophage phagocytosis and promoting tissue homeostasis. Conclusions Aging ovarian is ...