Antimicrobial and Anti-inflammatory Cyclic Tetrapeptides from the Co-cultures of Two Marine-Derived Fungi

作者：Qin Li, Shasha Li, Shunwang Li, Xiaomeng Hao, Anqi Wang, Shuyi Si, Yanni Xu, Jicheng Shu, Maoluo Gan · 发表于：Journal of Natural Products · 年份：2024 · DOI：10.1021/acs.jnatprod.3c01115 · 被引用次数：21 · 研究领域：Microbial Natural Products and Biosynthesis、Marine Sponges and Natural Products、Fungal Biology and Applications

Violaceotides B–E ( 1 – 4 ), four new cyclic tetrapeptides, along with seven known compounds, were identified from the sponge-associated Aspergillus insulicola IMB18-072 co-cultivated with the marine-derived Alternaria angustiovoidea IMB20-805. Their structures were elucidated by extensive analysis of spectroscopic data, including HRESIMS, 1D and 2D NMR, and MS/MS data. The absolute configurations were determined by the advanced Marfey’s method. Compounds 2, 3, and violaceotide A ( 5 ) displayed selective antimicrobial activities against the aquatic pathogenic bacteria Edwardsiella tarda and E. ictaluri . In addition, compounds 1 – 5 showed inhibitory activities against the LPS-induced expression of the inflammatory mediator IL-6 in RAW264.7 cells at a concentration of 10 μM.