Effects of flora deficiency on the structure and function of the large intestine

作者：Tailiang Chai, Juan Shen, Yifei Sheng, Yufen Huang, Weiming Liang, Zhao Zhang, Ruizhen Zhao, Haitao Shang, W. S. Cheng, Hang Zhang, Xueting Chen, Xiang Huang, Yin Zhang, Jiazhe Liu, Huanjie Yang, Linying Wang, Shanshan Pan, Yang Chen, Lijuan Han, Qinwei Qiu, Aibo Gao, Hong Wei, Xiaodong Fang · 发表于：iScience · 年份：2024 · DOI：10.1016/j.isci.2024.108941 · 被引用次数：10 · 研究领域：Gut microbiota and health、Gastrointestinal motility and disorders、Probiotics and Fermented Foods

The significant anatomical changes in large intestine of germ-free (GF) mice provide excellent material for understanding microbe-host crosstalk. We observed significant differences of GF mice in anatomical and physiological involving in enlarged cecum, thinned mucosal layer and enriched water in cecal content. Furthermore, integration analysis of multi-omics data revealed the associations between the structure of large intestinal mesenchymal cells and the thinning of the mucosal layer. Increased Aqp8 expression in GF mice may contribute to enhanced water secretion or altered hydrodynamics in the cecum. In addition, the proportion of epithelial cells, nutrient absorption capacity, immune function and the metabolome of cecum contents of large intestine were also significantly altered. Together, this is the first systematic study of the transcriptome and metabolome of the cecum and colon of GF mice, and these findings contribute to our understanding of the intricate interactions between microbes and the large intestine.