Multi-Omics Characteristics of Ferroptosis Associated with Colon Adenocarcinoma Typing and Survival

作者：Xiao‐Qiong Chen, Ke Lian, Ziwei Chen, Xuan Zhang, Ting Li, Tao Wu, Tao Shen, Xinyi Cai, Xianshuo Cheng, Fu‐Hui Xiao, Yunfeng Li · 发表于：Frontiers in Bioscience-Landmark · 年份：2024 · DOI：10.31083/j.fbl2901013 · 被引用次数：4 · 研究领域：Ferroptosis and cancer prognosis、Cancer-related molecular mechanisms research、Epigenetics and DNA Methylation

BACKGROUND: Ferroptosis, an iron-dependent form of cell death, plays a crucial role in the progression of various cancers, including colon adenocarcinoma (COAD). However, the multi-omics signatures relevant to ferroptosis regulation in COAD diagnosis remain to be elucidated. METHODS: The transcriptomic, miRNAomic, and methylomic profiles of COAD patients were acquired from the Cancer Genome Atlas (TCGA). Ferroptosis activity in these patients was determined, represented by a ferroptosis score (FS), using single-sample gene set enrichment analysis (ssGSEA) based on the expression of ferroptosis-related genes. RESULTS: Results showed that the COAD patients with high-FS displayed favorable survival outcomes and heightened drug sensitivity. They also exhibited an up-regulation of genes involved in immune-related pathways (e.g., tumor necrosis factor signaling pathway), suggesting a correlation between immunity and ferroptosis in COAD progression. Furthermore, three survival prediction models were established based on 10 CpGs, 12 long non-coding RNAs (lncRNAs), and 14 microRNAs (miRNAs), respectively. These models demonstrated high accuracy in predicting COAD survival, achieving areas under the curve (AUC) >0.7. The variables used in the three models also showed strong correlations at different omics levels and were effective at discriminating between high-FS and low-FS COAD patients (AUC >0.7). CONCLUSIONS: This study identified different DNA methylation (DNAm), lncRNA, and miRNA...