Genome-Wide Identification and Characterization of CHROMO Domain Family Genes Reveal Roles of the Maize Genes in Heat Stress Response

作者：Pengfei Duan, Yuanmeng Kan, Di Zhao, Shuqiong Yang, Jibao Chen · 发表于：Biology Bulletin · 年份：2023 · DOI：10.1134/s1062359022603494 · 被引用次数：1 · 研究领域：Photosynthetic Processes and Mechanisms、Plant Stress Responses and Tolerance、Genetic Mapping and Diversity in Plants and Animals

Abstract CMT2 and cpSRP43 are key factors in tolerance to heat stress, which have been revealed in Arabidopsis. It is largely unknown whether they play similar roles in heat stress resistance in monocotyledon species. Here we carried out genome-enabled identification of CHROMO domain genes in five monocots, to which both CMT2 and cpSRP43 belong. Results showed that gene copy numbers are similar in monocots to those in Arabidopsis. Phylogenetic analyses split the family into six major clades, among which CMT2 and cpSRP43 formed independent clades, respectively. Two CMT2s are present in the maize genome, while a single cpSRP43 exists in maize. Expression of the CTM2 homologs was elevated 2 and 48 h after heat stress in maize roots, while that of cpSRP43 was increased 2 and 48 h after stress in maize stalks. The genes identified here can be used as molecular targets for breeders to select for thermal-tolerant wheat lines, while phylogenetic and expression analyses of the CHROMO domain genes can be used as a knowledge base for molecular, biochemical and physiological characterization of the genes in monocots.