Traceability of Geographic Origin Using Human Skin and Oral Microbiota.

作者：Xin-Yu Dong, Ruxin Zhu, Yinlei Lei, Ruiyang Tao, Chengtao Li · 发表于：PubMed · 年份：2023 · DOI：10.12116/j.issn.1004-5619.2023.530401 · 被引用次数：2 · 研究领域：Forensic and Genetic Research、Oral microbiology and periodontitis research、Forensic Anthropology and Bioarchaeology Studies

OBJECTIVES: To explore the possibility of using human skin and oral microorganisms to estimate the geographic origin of an individual through the sequencing analysis of bacterial 16S rRNA gene. METHODS: Microbial DNA was extracted from the palm and oral microorganisms of the Han population in Shanghai and Chifeng, Inner Mongolia, and the composition and diversity of the microbiota were analyzed by full-length 16S rRNA gene sequencing. Then, differential species were screened and a geographic location prediction model was constructed. RESULTS: ) for skin and oral samples were 0.129 and 0.102, respectively. Through principal co-ordinates analysis (PCoA), the samples from the two places could be preliminarily distinguished. The predictive model had the accuracies of 0.90 and 0.83 for the geographic origin using the skin and oral samples, respectively. CONCLUSIONS: There are differences in the compositions of palm and oral microbiota between Han populations in Shanghai and Chifeng. The prediction model constructed by the random forest algorithm can trace the unknown individuals from the above two places.