Identifying animal-derived components in camel milk and its products by ultra-high performance liquid chromatography-tandem mass spectrometry

作者：Shuqing Gu, Niannian Chen, Jing Zeng, Xiaoyu Peng, Min Zhang, Yu Gao, Lina Pan, Ge Cheng, Wei Li, Xionghai Yi, Dehua Guo, Xiaojun Deng · 发表于：Chinese Journal of Chromatography · 年份：2024 · DOI：10.3724/sp.j.1123.2023.07027 · 被引用次数：4 · 研究领域：Animal Diversity and Health Studies、Identification and Quantification in Food、Meat and Animal Product Quality

A method for identifying specific peptide biomarkers of animal-milk-derived components in camel milk and its products was established using proteomics. Samples were prepared by defatting, protein extraction, and trypsin hydrolysis, and proteins and peptides were identified using ultra-high performance liquid chromatography-quadrupole/electrostatic orbitrap-high resolution mass spectrometry (UHPLC-Q/Exactive-HRMS) and Protein Pilot software. Twenty two peptide biomarkers from eight species (i.e., Camelus , Bos taurus , Bubalus bubalis , Bos grunniens/Bos mutus , Capra hircus , Ovis aries , Equus asinus , Equus caballus ) were identified by comparing the basic local alignment search tool (BLAST) with the Uniprot database. Verification of these marker peptides were performed quantitatively using a UHPLC-triple-quadrupole mass-spectrometry (QqQ-MS) system by multiple reaction monitoring (MRM). The pretreatment method of casein in camel milk was optimized, such as defatting, protein precipitation, and re-dissolving buffer solution. The effects of various mass-spectrometry parameters, such as atomization gas, heating- and drying-gas flow rates, and desolvation-tube (DL) and ion-source-interface temperatures on ion-response intensity were optimized. Camel milk signature peptides were detected in a mixture of milk from other seven species to ensure specificity for the selected biomarker peptides. The signature peptides of seven other species were also detected in camel milk. ...