Microsporidia dressing up: the spore polaroplast transport through the polar tube and transformation into the sporoplasm membrane

作者：Qing Lv, Liuyi Hong, Lei Qi, Yuqing Chen, Zhengkai Xie, Hongjie Liao, Chunfeng Li, Tian Li, Xianzhi Meng, Jie Chen, Jialing Bao, Junhong Wei, Bing Han, Qingtao Shen, Louis M. Weiss, Zeyang Zhou, Mengxian Long, Guoqing Pan · 发表于：mBio · 年份：2024 · DOI：10.1128/mbio.02749-23 · 被引用次数：23 · 研究领域：Parasitic Infections and Diagnostics、Plant Disease Resistance and Genetics、Plant Virus Research Studies

sporoplasm surface proteins (NbTMP1 and NoboABCG1.1) were also found to localize in the region of the polaroplast and posterior vacuole in mature spores and in the discharged polar tube, which suggested that the polaroplast during transport through the polar tube became the limiting membrane of the sporoplasm. The analysis results of Golgi-tracker green and Golgi marker protein syntaxin 6 were also consistent with the model of the transported polaroplast derived from Golgi transformed into the nascent sporoplasm membrane.IMPORTANCEMicrosporidia, which are obligate intracellular pathogenic organisms, cause huge economic losses in agriculture and even threaten human health. The key to successful infection by the microsporidia is their unique invasion apparatus which includes the polar filament, polaroplast, and posterior vacuole. When the mature spore is activated to geminate, the polar filament uncoils and undergoes a rapid transition into the hollow polar tube that transports the sporoplasm components including the microsporidian nucleus into host cells. Details of the structural difference between the polar filament and polar tube, the process of cargo transport in extruded polar tube, and the formation of the sporoplasm membrane are still poorly understood. Herein, we verify that the polar filament evaginates to form the polar tube, which serves as a conduit for transporting the nucleus and other sporoplasm components. Furthermore, our results indicate that the transported ...