The morphokinetic signature of human blastocysts with mosaicism and the clinical outcomes following transfer of embryos with low-level mosaicism

作者：Yaoyu Zou, Yilun Sui, Jing Fu, Naidong Ge, Xiaoxi Sun, Yijuan Sun · 发表于：Journal of Ovarian Research · 年份：2024 · DOI：10.1186/s13048-023-01324-w · 被引用次数：12 · 研究领域：Prenatal Screening and Diagnostics、Reproductive Biology and Fertility、Assisted Reproductive Technology and Twin Pregnancy

Abstract Background Genetic mosaicism is commonly observed in human blastocysts. Embryos’ morphokinetic feature observed from time-lapse monitoring (TLM) is helpful to predict the embryos’ ploidy status in a non-invasive way. However, morphokinetic research on mosaic embryos is extremely limited. Moreover, transfer of mosaic embryos is a new attempt in reproductive medicine, while studies regarding the clinical and neonatal outcomes following transfer of embryos with different levels and types of mosaicism are needed. This study aimed to investigate the morphokinetic characteristics of mosaic blastocysts, uncover clinical outcomes of mosaic embryos, and evaluate the effect of level and type of mosaicism on transfer outcomes. Results A total of 923 blastocysts from 229 preimplantation genetic testing cycles were cultured in TLM incubators in a single fertilization center between July 2016 and July 2021. Multivariate logistic regression models showed mosaic embryos had significantly shorter time to reach morula when compared with euploid ( P = 0.002), mosaic with aneuploid ( P = 0.005), and aneuploid ( P = 0.005) embryos after adjusting the potential confounders. KIDScore is an artificial intelligence scoring program from time lapse incubation system to predict embryo implantation potential. Mosaic with aneuploid embryos had significantly lower KIDScore than euploid ( P = 6.47e −4 ), mosaic ( P = 0.005), and aneuploid ( P = 0.004) embryos after adjustment. Meanwhile, we compare...