Embedded bioprinted multicellular spheroids modeling pancreatic cancer bioarchitecture towards advanced drug therapy

作者：Xiaoyun Wei, Yiwen Wu, Keke Chen, Ling Wang, Mingen Xu · 发表于：Journal of Materials Chemistry B · 年份：2024 · DOI：10.1039/d3tb02913a · 被引用次数：20 · 研究领域：3D Printing in Biomedical Research、Innovative Microfluidic and Catalytic Techniques Innovation、Cancer Cells and Metastasis

self assembly. By modulating the printing parameters, SMAs, even from multiple cell components, could be manipulated with tunable size and flexible location, achieving tunable spheroid patterns within the hydrogel bath with reproducible morphological features. To demonstrate the feasibility of this printing strategy, we fabricated desmoplastic PDAC spheroids by printing SMAs consisting of tumor cells and fibroblasts within the GelMA matrix bath. The produced hybrid spheroids were further exposed to different concentrations of the drug gemcitabine to verify their potential for use in cell therapy. Beyond providing a robust and facile bioprinting system that enables desmoplastic PDAC bioarchitecture bioengineering, this work introduces an approach for the scalable, flexible and rapid fabrication of cell spheroids or multi-cell-type spheroid patterns as platforms for advanced drug therapy or disease mechanism exploration.