Clinical Significance of Disulfidptosis-related Genes and Functional Analysis in Gastric Cancer

作者：Jianing Yan, Ziyi Fang, Meiqi Shi, Can Tu, Shengke Zhang, Cheng‐Lu Jiang, Qier Li, Yongfu Shao · 发表于：Journal of Cancer · 年份：2024 · DOI：10.7150/jca.91796 · 被引用次数：20 · 研究领域：Ferroptosis and cancer prognosis、Cancer-related molecular mechanisms research、RNA modifications and cancer

Background: Worldwide, gastric cancer (GC) remains intractable due to its poor prognosis and high morbidity and mortality.Disulfidptosis is a novel kind of cell death mediated by abnormal accumulation of intracellular disulphides.The correlation between disulfidptosis and GC is still unknown.Therefore, it is necessary to elucidate the pathogenesis and mechanism of disulfidptosis and GC for clinical diagnosis and intervention.Methods: RNA-sequencing data from several public data portals and clinical samples were collected.We compared the expression levels of four key genes of disulfidptosis, including SLC7A11, SLC3A2, RPN1, and NCKAP1, in GC and selected prognostic genes to build a novel GC prognosis-related nomogram model.The biological functions and immune landscape of the identified prognostic genes were explored.Results: Overexpressed NCKAP1 and SLC7A11 were prognostic disulfidptosis-related genes in GC.We combined these genes and several clinicopathological factors to build a prognostic nomogram model for GC.Meanwhile, the ROC curves showed that NCKAP1 and SLC7A11 were promising biomarkers for GC screening.The biological and cellular functions were focused on actin activities, GTPase and immunoreaction.The tumour immune microenvironment and immune therapy targets were identified.Competing endogenous RNA network was built to explore the downstream regulatory mechanisms.Finally, the elevated NCKAP1 and SLC7A11 expression in GC was validated via qRT-PCR in a cell line and ti...