Impact of ferroptosis-related risk genes on macrophage M1/M2 polarization and prognosis in glioblastoma

作者：Xin Xu, Yue Zhang, Chenlong Liao, Han Zhou, Yiwei Wu, Wenchuan Zhang · 发表于：Frontiers in Cellular Neuroscience · 年份：2024 · DOI：10.3389/fncel.2023.1294029 · 被引用次数：13 · 研究领域：Ferroptosis and cancer prognosis、Immune cells in cancer、Glioma Diagnosis and Treatment

Objective: To explore the effect impact of ferroptosis on macrophage polarization and patient prognosis in glioblastoma. Methods: We screened ferroptosis-related risk from the public datasets of primary and recurrent glioblastoma, combined with reported ferroptosis genes, calculated the risk genes among the ferroptosis-related genes using the LASSO Cox regression model, and investigated the relationship between these ferroptosis-related risk genes in the tumor and the spectrum of infiltrating M1/M2 macrophages. Macrophages were analyzed using the CIBERSORTx deconvolution algorithm. Samples from The Cancer Genome Atlas (TCGA), Chinese Glioma Genome Atlas (CGGA) and a single-cell RNA sequencing dataset (GSE84465) were included. The expression levels of ferroptosis-related risk genes and molecular markers of M1 and M2 macrophages were detected by qPCR and western blot. Results: A total of fourteen ferroptosis-related risk genes were obtained and the patients' risk scores were calculated. Compared with patients in the low-risk group, patients in the high-risk group had worse prognosis. The M1/M2 macrophage ratio and risk score were negatively correlated, indicating that the tumor microenvironment of glioblastoma in the high-risk group contained more M2 than M1 macrophages. In the single-cell RNA sequencing dataset, the risk score of ferroptosis-related genes in tumor cells was positively correlated with the proportion of high M2 macrophages. The expression of eight ferroptosis-re...