Stretch response of the mechano-gated channel TMEM63A in membrane patches and single cells

作者：Sayeman Islam Niloy, Peter R. Strege, Elizabeth Hannan, Luke M. Cowan, Fabian Linsenmeier, Oliver Friedrich, Gianrico Farrugia, Arthur Beyder · 发表于：American Journal of Physiology-Cell Physiology · 年份：2024 · DOI：10.1152/ajpcell.00583.2023 · 被引用次数：9 · 研究领域：Erythrocyte Function and Pathophysiology、Ion channel regulation and function、Connexins and lens biology

TMEM63A has biophysical features distinctive for mechano-gated cation channels, but some properties are less clear, including no functional information in whole cells. We report that pressure-dependent yet voltage-independent TMEM63A currents in cell membrane patches correlated with cell size. In addition, radial stretch of whole cells on flexible membranes increased the [Ca 2+ ] i responses more in TMEM63A-transfected cells. Inward TMEM63A currents in response to high stretch can depolarize plasma membranes and contribute to a sustained [Ca 2+ ] i increase.