Nitric oxide mediates red light-induced perylenequinone production in Shiraia mycelium culture

作者：Wen Juan Wang, Xin Ping Li, Wen Hao Shen, Qun Yan Huang, Rui Peng Cong, Li Ping Zheng, Jianwen Wang · 发表于：Bioresources and Bioprocessing · 年份：2024 · DOI：10.1186/s40643-023-00725-5 · 被引用次数：9 · 研究领域：Algal biology and biofuel production、Biocrusts and Microbial Ecology、Fungal Biology and Applications

Abstract Perylenequinones (PQs) from bambusicolous Shiraia fungi serve as excellent photosensitizers for photodynamic therapy. However, the lower yield of PQ production in mycelium cultures is an important bottleneck for their clinical application. Light has long been recognized as a pivotal regulatory signal for fungal secondary metabolite biosynthesis. In this study, we explored the role of nitric oxide (NO) in the growth and PQ biosynthesis in mycelium cultures of Shiraia sp. S9 exposed to red light. The continuous irradiation with red light (627 nm, 200 lx) suppressed fungal conidiation, promoted hyphal branching, and elicited a notable increase in PQ accumulation. Red light exposure induced NO generation, peaking to 81.7 μmol/g FW on day 8 of the culture, with the involvement of nitric oxide synthase (NOS)- or nitrate reductase (NR)-dependent pathways. The application of a NO donor sodium nitroprusside (SNP) restored conidiation of Shiraia sp. S9 under red light and stimulated PQ production, which was mitigated upon the introduction of NO scavenger carboxy-PTIO or soluble guanylate cyclase inhibitor NS-2028. These results showed that red light-induced NO, as a signaling molecule, was involved in the regulation of growth and PQ production in Shiraia sp. S9 through the NO-cGMP-PKG signaling pathway. While mycelial H 2 O 2 content exhibited no significant alternations, a transient increase of intracellular Ca 2+ and extracellular ATP (eATP) content was detected upon exposur...