An Optimal Genomic Dna Extraction Method for Shoots of Four Dendrocalamus Species Based on Membership Function Analysis

作者：Yunlong Ma, Bowen Li, Dejia Yang, Shuguang Wang, Lixia Yu, Hui Zhan, Juan Li · 发表于：BioTechniques · 年份：2023 · DOI：10.2144/btn-2023-0087 · 被引用次数：7 · 研究领域：Bamboo properties and applications、Chromosomal and Genetic Variations、Agricultural Systems and Practices

High-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that the commercial DNA extraction kit method was the most effective and comprehensive approach for extracting genomic DNA from shoots of four Dendrocalamus species. Furthermore, this study provided valuable insights into optimizing techniques for extracting genomic DNA in other bamboo species.