A Sirtuin-Dependent T7 RNA Polymerase Variant

作者：Yongan Wang, Yanli Ji, Lin Sun, Zhifen Huang, Sheng Ye, Weimin Xuan · 发表于：ACS Synthetic Biology · 年份：2023 · DOI：10.1021/acssynbio.3c00607 · 被引用次数：1 · 研究领域：Sirtuins and Resveratrol in Medicine、CRISPR and Genetic Engineering、Virus-based gene therapy research

Transcriptional regulation is of great significance for cells to maintain homeostasis and, meanwhile, represents an innovative but less explored means to control biological processes in synthetic biology and bioengineering. Herein we devised a T7 RNA polymerase (T7RNAP) variant through replacing an essential lysine located in the catalytic core (K631) with N ε -acetyl- l -lysine (AcK) via genetic code expansion. This T7RNAP variant requires the deacetylase activity of NAD-dependent sirtuins to recover its enzymatic activities and thereby sustains sirtuin-dependent transcription of the gene of interest in live cells including bacteria and mammalian cells as well as in in vitro systems. This T7RNAP variant could link gene transcription to sirtuin expression and NAD availability, thus holding promise to support some relevant research.