Unlocking the potential of glyphosate-resistant bacterial strains in biodegradation and maize growth

作者：Waqas Mohy-Ud-Din, Feng Chen, Safdar Bashir, Muhammad Javed Akhtar, Hafiz Naeem Asghar, Zia Ur Rahman Farooqi, Usman Zulfiqar, Fasih Ullah Haider, Aneeqa Afzal, Mashael Daghash Alqahtani · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1285566 · 被引用次数：13 · 研究领域：Pesticide and Herbicide Environmental Studies、Plant tissue culture and regeneration、Legume Nitrogen Fixing Symbiosis

Glyphosate [N-(phosphonomethyl)-glycine] is a non-selective herbicide with a broad spectrum activity that is commonly used to control perennial vegetation in agricultural fields. The widespread utilization of glyphosate in agriculture leads to soil, water, and food crop contamination, resulting in human and environmental health consequences. Therefore, it is imperative to devise techniques for enhancing the degradation of glyphosate in soil. Rhizobacteria play a crucial role in degrading organic contaminants. Limited work has been done on exploring the capabilities of indigenously existing glyphosate-degrading rhizobacteria in Pakistani soils. This research attempts to discover whether native bacteria have the glyphosate-degrading ability for a sustainable solution to glyphosate contamination. Therefore, this study explored the potential of 11 native strains isolated from the soil with repeated glyphosate application history and showed resistance against glyphosate at higher concentrations (200 mg kg −1 ). Five out of eleven strains outperformed in glyphosate degradation and plant growth promotion. High-pressure liquid chromatography showed that, on average, these five strains degraded 98% glyphosate. In addition, these strains promote maize seed germination index and shoot and root fresh biomass up to 73 and 91%, respectively. Furthermore, inoculation gave an average increase of acid phosphatase (57.97%), alkaline phosphatase (1.76-fold), and dehydrogenase activity (1.75-fol...