Sporosarcina siccatus sp. nov., isolated from coastal sediment of the Great Wall Station, Antarctica

作者：Hongmei Mu, Yuchun Xie, Peiqiang Jiang, Lukuan Liu, Jing Li · 发表于：Research Square · 年份：2023 · DOI：10.21203/rs.3.rs-3765548/v1 · 研究领域：Genomics and Phylogenetic Studies、Polar Research and Ecology、Microbial Community Ecology and Physiology

Abstract A Gram-stain-positive, aerobic and spherical bacterium, white, slightly convex colony with rounded edges, designated GW1-11 T was isolated from the coastal sediment of the Great Wall Station, Antarctica. 16S rRNA gene sequence analysis showed that strain GW1-11 T was closely related to Sporosarcina ureae DSM 2281 T (94%). Growth was observed in the presence of 0–6.0%(w/v) NaCl, at pH 6.0–9.0, and at 4–37℃. Digital DNA-DNA relatedness between strain GW1-11 T and its close relatives S. ureae DSM 2281 T was 21.0%. The average nucleotide identity value between strain GW1-11 T and S. ureae DSM 2281 T was 77.9%. The strain GW1-11 T possessed menaquinone-7 (MK-7) as the sole respiratory quinone. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The major fatty acids composition was summarized as C 14: 0 , C 14:0 ω 5 c and iso -C 14: 0 . Based on phenotypic, chemotaxonomic characteristics, and phylogenetic data, strain GW1-11 T represents a novel species of the genus Sporosarcina , for which the name Sporosarcina siccatus sp. nov., is proposed. The type strain is GW1-11 T (= CCTCC AB 2019391 T = KCTC43187 T ).