Immunohistochemical examination of the uteroplacental interface of cows on days 21, 31, 40, and 67 of gestation

作者：Heewon Seo, Gabriela Dalmaso de Melo, Ramiro V Oliveira, Gessica A Franco-Johannsen, Fuller W. Bazer, Ky G Pohler, Gregory A. Johnson · 发表于：Reproduction · 年份：2023 · DOI：10.1530/rep-23-0444 · 被引用次数：21 · 研究领域：Reproductive Physiology in Livestock、Reproductive Biology and Fertility、Animal health and immunology

In brief: Cattle are classified as having synepitheliochorial placentation in which the majority of the uterine luminal epithelial cells remain intact with some luminal epithelial cells fusing with binucleate trophoblast cells to form syncytial trinucleate cells. This study suggests the possibility that, for a limited and as yet undefined period of gestation, the majority of luminal epithelial cells are eliminated and replaced by trophoblast cells that express pregnancy-associated glycoproteins. Abstract: What we understand about the early stages of placentation in cattle is based on an elegant series of electron microscopic images that provide exquisite detail but limited appreciation for the microanatomy across the uteroplacental interface. In order to achieve a global perspective on the histology of bovine placentation during critical early stages of gestation, i.e., days 21, 31, 40, and 67, we performed immunohistochemistry to detect cell-specific expression of pregnancy-associated glycoprotein (PAG), cytokeratin, epithelial (E)-cadherin, and serine hydroxymethyltransferase 2 (SHMT2) at the intact uteroplacental interface. Key findings from the immunohistochemical analyses are that there are: (i) PAG-positive cells with a single nucleus within the uterine luminal epithelial (LE) cells; (ii) PAG-positive cells with two nuclei in the LE; (iii) PAG-positive syncytial cells with more than three nuclei in the LE; (iv) LE cells that are dissociated from one another and from the...