MEMS-based two-photon microscopy with Lissajous scanning and image reconstruction under a feed-forward control strategy

作者：Xiulei Zhang, Yongxuan Han, huilan liu, Xilong Xiao, Yanhui Hu, Qiang Fu, Lishuang Feng, Xiaoguang Hu, Conghao Wang, Junjie Wang, Aimin Wang · 发表于：Optics Express · 年份：2023 · DOI：10.1364/oe.510979 · 被引用次数：5 · 研究领域：Advanced Fluorescence Microscopy Techniques、Force Microscopy Techniques and Applications、Image Processing Techniques and Applications

Two-photon microscopy (TPM) based on two-dimensional micro-electro-mechanical (MEMS) system mirrors shows promising applications in biomedicine and the life sciences. To improve the imaging quality and real-time performance of TPM, this paper proposes Lissajous scanning control and image reconstruction under a feed-forward control strategy, a dual-parameter alternating drive control algorithm and segmented phase synchronization mechanism, and pipe-lined fusion-mean filtering and median filtering to suppress image noise. A 10 fps frame rate (512 × 512 pixels), a 140 µm × 140 µm field of view, and a 0.62 µm lateral resolution were achieved. The imaging capability of MEMS-based Lissajous scanning TPM was verified by ex vivo and in vivo biological tissue imaging.