Synergistic horizontal transfer of antibiotic resistance genes and transposons in the infant gut microbial genome

作者：Yanwen Ding, Xin Jiang, Jiacheng Wu, Yihui Wang, Lanlan Zhao, Yingmiao Pan, Yaxuan Xi, Guoping Zhao, Ziyun Li, Lei Zhang · 发表于：mSphere · 年份：2023 · DOI：10.1128/msphere.00608-23 · 被引用次数：11 · 研究领域：Antibiotic Resistance in Bacteria、Gut microbiota and health、Clostridium difficile and Clostridium perfringens research

ABSTRACT Transposons, plasmids, bacteriophages, and other mobile genetic elements facilitate horizontal gene transfer in the gut microbiota, allowing some pathogenic bacteria to acquire antibiotic resistance genes (ARGs). Currently, the relationship between specific ARGs and specific transposons in the comprehensive infant gut microbiome has not been elucidated. In this study, ARGs and transposons were annotated from the Unified Human Gastrointestinal Genome (UHGG) and the Early-Life Gut Genomes (ELGG). Association rules mining was used to explore the association between specific ARGs and specific transposons in UHGG, and the robustness of the association rules was validated using the external database in ELGG. Our results suggested that ARGs and transposons were more likely to be relevant in infant gut microbiota compared to adult gut microbiota, and nine robust association rules were identified, among which Klebsiella pneumoniae , Enterobacter hormaechei_A , and Escherichia coli_D played important roles in this association phenomenon. The emphasis of this study is to investigate the synergistic transfer of specific ARGs and specific transposons in the infant gut microbiota, which can contribute to the study of microbial pathogenesis and the ARG dissemination dynamics. IMPORTANCE The transfer of transposons carrying antibiotic resistance genes (ARGs) among microorganisms accelerates antibiotic resistance dissemination among infant gut microbiota. Nonetheless, it is unclear w...