Biodegradation of Cholesterol by Enterococcus faecium YY01

作者：Yang Rui-min, Shahbaz Ahmad, Hongyan Liu, Qianqian Xu, Chunhua Yin, Yang Liu, Haiyang Zhang, Hai Yan · 发表于：Microorganisms · 年份：2023 · DOI：10.3390/microorganisms11122979 · 被引用次数：9 · 研究领域：Drug Transport and Resistance Mechanisms、Cholesterol and Lipid Metabolism、Helicobacter pylori-related gastroenterology studies

Cholesterol (CHOL) is one of the risk factors causing the blockage of the arterial wall, atherosclerosis, coronary heart disease, and other serious cardiovascular diseases. Here, a promising bacterial strain for biodegrading CHOL was successfully isolated from the gut of healthy individuals and identified as Enterococcus faecium YY01 with an analysis of the 16S rDNA sequence. An initial CHOL of 1.0 g/L was reduced to 0.5 g/L in 5 days, and glucose and beef extract were found to be optimal carbon and nitrogen sources for the rapid growth of YY01, respectively. To gain further insight into the mechanisms underlying CHOL biodegradation, the draft genome of YY01 was sequenced using Illumina HiSeq. Choloylglycine hydrolase, acyltransferase, and alkyl sulfatase was encoded by gene0586, gene1890, and gene2442, which play crucial roles in converting 3α, 7α, 12α-trihydroxy-5β-choranic acid to choline-CoA and then choline-CoA to bile acid. Notably, choloylglycine hydrolase was closely related to the biosynthesis of both primary and secondary bile acid. The findings of this study provide valuable insights into the metabolism pathway of CHOL biodegradation by YY01 and offer a potential avenue for the development of bacterioactive drugs against hypercholesterolemia.