Biodegradation of Imazethapyr by Bacterial Strain IM9601 Isolated from Agricultural Soil

作者：Zehua Xu, Baiyun Li, Yonghua Jia, Xinnian Guo, Fanyang Lv · 发表于：Current Microbiology · 年份：2023 · DOI：10.1007/s00284-023-03533-4 · 被引用次数：5 · 研究领域：Pesticide and Herbicide Environmental Studies、Pharmaceutical and Antibiotic Environmental Impacts、Microbial bioremediation and biosurfactants

Abstract The widespread utilization of the herbicide imazethapyr presents significant challenges to crop rotation and results in detrimental soil degradation issues. Bacterial biodegradation has emerged as a promising and eco-friendly approach for mitigating pesticide residues contamination in the environment. In this study, a novel bacterium, identified as Brevibacterium sp. IM9601, was isolated and characterized based on morphological, physiological, and biochemical characteristics, as well as 16S rRNA gene sequence. This strain exhibited the ability to utilize imazethapyr as its sole carbon source for growth. Response surface methodology (RSM) was applied to optimize the degradation conditions. The most favorable conditions were determined to be a temperature of 27 °C, pH of 6.0, and an initial inoculum with a final OD 600 of 0.15. Under these optimized condition, bacterial strain IM9601 exhibited substantial imazethapyr degradation, with removal rates of 90.08 and 87.05% for initial imazethapyr concentrations of 50 and 100 mg L −1 , respectively, achieved within a 5-day incubation period. This investigation highlights imazethapyr-degrading capabilities of the Brevibacterium genus bacterial strain IM9601, marking it as a potentially novel and effective solution for addressing the environmental pollution resulting from the usage of imazethapyr. The study contributes to the growing body of research on bioremediation approaches, offering a sustainable and environmentally frie...